HEPES CAS:7365-45-9 Product Introduction
Product name: 2-[4-(2-hydroxyerhyl)-1-piperazinyl]ethanesulfonic acid (HEPES)
Appearance: White powder.
4-Hydroxyethylpiperazineethanesulfonic acid (HEPES) is an important hydrogen ion buffer, whichhas a good buffering capacity in the range of pH 6.8-8.2, and can control a constant Chemicalbook pH for a long time. The use concentration is 10-50 mmol/L. Generally, the culture medium contains 20 mmol/L of 4-hydroxyethylpiperazineethanesulfonic acid, which can have good buffering ability and has no toxic effect on cells.
Specificatin Properties Standard
Appearance White crystal powder
Loss on drying，%
Uv absorbance(260nm,1M aq.)
Uv absorbance(280nm,1M aq.)
1.As biochemical reagent
2.Biological buffers; reaction buffers, prehybridization buffers, hybridization buffers for isolation and analysis of RNA nuclear fractions; for RNA and T4RNA
3.Molecular biology grade is used for RNA 3′-end labeling with T4 RNA ligase Chemicalbook, and the components of reaction buffer, pre-hybridization buffer and hybridization buffer for separation and analysis of nuclear RNA
4.Cell-cell adhesion, short-term cell aggregation culture, washing buffers for tissues and cells
– 25kg moisture-proof woven bag lined with two layers of plastic bags,
– 1000kg moisture-proof big bag lined with two layers of plastic bags.
Storage and transportation:
Should be stored in a dry, cool, well-ventilated place. To prevent sun exposure, isolate from fire and heat sources, and do not store and transport with ammonia, oxygen, phosphorus, antimony powder and alkalis. It should be kept away from sawdust, shavings, straw, etc. to prevent burning
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