HEPES CAS:7365-45-9 Product Introduction
Product name: 2-[4-(2-hydroxyerhyl)-1-piperazinyl]ethanesulfonic acid (HEPES)
CAS: 7365-45-9
MF: C8H18N2O4S
MW: 238.3
Appearance: White powder.
Characteristic:
4-Hydroxyethylpiperazineethanesulfonic acid (HEPES) is an important hydrogen ion buffer, whichhas a good buffering capacity in the range of pH 6.8-8.2, and can control a constant Chemicalbook pH for a long time. The use concentration is 10-50 mmol/L. Generally, the culture medium contains 20 mmol/L of 4-hydroxyethylpiperazineethanesulfonic acid, which can have good buffering ability and has no toxic effect on cells.
分析项目 技术指标 Specificatin Properties Standard |
实测结果 Results |
外观 白色结晶粉末 Appearance White crystal powder |
符合 Conforms |
含量 ≥99 Assay,% |
100.1 |
干燥失重 ≤0.5 Loss on drying,% |
0.22 |
重金属 ≤5 Heavy metals,ppm |
符合 Conforms |
PH(1%水溶液) 5.0-6.5 PH(1% aq.) |
5.1 |
紫外吸光度/260nm(1M 水溶液) ≤0.1 Uv absorbance(260nm,1M aq.) |
0.07 |
紫外吸光度/280nm(1M 水溶液) ≤0.07 Uv absorbance(280nm,1M aq.) |
0.06 |
灼烧残渣 ≤0.1 Ignition residue,% |
0.01 |
HEPES Application:
1.As biochemical reagent
2.Biological buffers; reaction buffers, prehybridization buffers, hybridization buffers for isolation and analysis of RNA nuclear fractions; for RNA and T4RNA
3.Molecular biology grade is used for RNA 3′-end labeling with T4 RNA ligase Chemicalbook, and the components of reaction buffer, pre-hybridization buffer and hybridization buffer for separation and analysis of nuclear RNA
4.Cell-cell adhesion, short-term cell aggregation culture, washing buffers for tissues and cells
Package:
– 25kg moisture-proof woven bag lined with two layers of plastic bags,
– 1000kg moisture-proof big bag lined with two layers of plastic bags.
Storage and transportation:
Should be stored in a dry, cool, well-ventilated place. To prevent sun exposure, isolate from fire and heat sources, and do not store and transport with ammonia, oxygen, phosphorus, antimony powder and alkalis. It should be kept away from sawdust, shavings, straw, etc. to prevent burning
Related Products——biological buffer
7365-45-9 | HEPES |
1132-61-2 | 3-Morpholinopropanesulfonic acid |
4432-31-9 | 4-Morpholineethanesulfonic acid |
16052-06-5 | EPPS |
5625-37-6 | PIPES |
77-86-1 | Trometamol |
29915-38-6 | TAPS |
1135-40-6 | N-Cyclohexyl-3-aminopropanesulfonic acid |
10191-18-1 | BES |
7365-44-8 | 2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid |
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